CN
| 产品货号 | 产品类型 | 装载载体 | 基因信息 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| TY-VRW0575 | 乳酸杆菌表达载体 | pNZ8148 | pNZ8148 | 300 | 现货 |
pNZ8148
| 名称 | pNZ8148 | 别称 | |
|---|---|---|---|
| 基因长 | 质粒宿主 | 乳酸菌 | |
| 质粒用途 | 基因表达 | 片段类型 | ORF |
| 片段物种 | 空载体 | 原核抗性 | Chl |
| 筛选标记 | Chl | 荧光标记 | |
| 启动子 | PnisA | 复制子 | repA |
| 感受态 | MC1061 | 温度 | 30度 |
| 背景载体 | 正向引物 | ||
| 反向引物 | 拷贝数 | 低 | |
| 诱导方式 |
产品参数
启动子：PnisA promoter
复制子：repA，repC
终止子：T
质粒分类：乳酸杆菌表达载体；乳酸乳球菌表达载体
质粒大小：3167 bp
原核抗性：Chl+（氯霉素），工作浓度为25ug/ml
克隆菌株：MC1061
培养温度：37℃
表达宿主：NZ9000乳酸乳球菌
备注：注意pNZ8148转化扩增、克隆构建时必须使用MC1061感受态。您可以从HonorGene购买获得MC1061菌株。
载体介绍
The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII.
The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus.
pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).
产品说明书
载体序列
