pT3-myr-AKT-HA
(Plasmid #31789)

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• Depositing Lab

  Xin Chen

• Publication

  Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-83. Epub 2010 Dec 11.( How to cite )

• Sequence Information

• Sequences (3)

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This material is available to academics and nonprofits only.

Backbone

• Vector backbone

  pT3-EF1a

• Backbone size w/o insert (bp)7000

• Vector type

  Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s)

  Ampicillin, 100 μg/mL

• Growth Temperature

  37°C

• Growth Strain(s)

  DH5alpha

• Copy number

  Unknown

Gene/Insert

• Gene/Insert name

  Akt1

• Alt name

  Akt

• Species

  M. musculus (mouse)

• Insert Size (bp)

  1503

• Entrez Gene

  Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)

• Tags / Fusion Proteins

• Myr (N terminal on insert)

• HA (C terminal on insert)

Cloning Information

• Cloning methodGateway Cloning

• 5′ sequencing primerEF-1a-Fwd

• 3′ sequencing primerpT3EF1a-R 5'-TGACACCTACTCAGACAATG

• (Common Sequencing Primers)

Resource Information

• Articles Citing this Plasmid

• 44 References

Terms and Licenses

• Academic/Nonprofit Terms

• UBMTA

• Industry Terms

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.