pDC316-mCMV-EGFP载体质粒基本信息

pDC316-mCMV-EGFP质粒图谱载体图谱和pDC316-mCMV-EGFP载体序列质粒序列多克隆位点信息

pDC316-mCMV-EGFP质粒载体简介

pDC316-mCMV-EGFP质粒序列载体序列

LOCUS       pDC316-mCMV-EGFP                5879 bp ds-DNA     circular SYN 10-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
    source          1..5879
                    /organism="synthetic DNA construct"
                    /mol_type="other DNA"
    misc_feature    468..992
                    /label=mCMV promoter
    CDS             999..1715
                    /codon_start=1
                    /product="enhanced GFP"
                    /label=EGFP
                    /note="mammalian codon-optimized"
                    /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                    KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                    GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                    VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                    EFVTAAGITLGMDELYK"
    polyA_signal    1862..1983
                    /label=SV40 poly(A) signal
                    /note="SV40 polyadenylation signal"
    enhancer        1991..2370
                    /label=CMV enhancer
                    /note="human cytomegalovirus immediate early enhancer"
    promoter        2371..2574
                    /label=CMV promoter
                    /note="human cytomegalovirus (CMV) immediate early
                    promoter"
    promoter        2619..2637
                    /label=T7 promoter
                    /note="promoter for bacteriophage T7 RNA polymerase"
    misc_feature    2651..2740
                    /label=MCS
    polyA_signal    2764..2988
                    /label=bGH poly(A) signal
                    /note="bovine growth hormone polyadenylation signal"
    polyA_signal    3002..3136
                    /label=SV40 poly(A) signal
                    /note="SV40 polyadenylation signal"
    protein_bind    3156..3189
                    /label=loxP
                    /bound_moiety="Cre recombinase"
                    /note="Cre-mediated recombination occurs in the 8-bp core
                    sequence (GCATACAT)."
    rep_origin      complement(3613..4201)
                    /direction=LEFT
                    /label=ori
                    /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                    replication"
    CDS             complement(4372..5232)
                    /codon_start=1
                    /gene="bla"
                    /product="beta-lactamase"
                    /label=AmpR
                    /note="confers resistance to ampicillin, carbenicillin, and
                    related antibiotics"
                    /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                    ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                    PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                    EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                    LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                    LIKHW"
    promoter        complement(5233..5337)
                    /gene="bla"
                    /label=AmpR promoter
    misc_feature    5420..453
                    /label=Ad
ORIGIN
       1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
      61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
     121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
     181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
     241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
     301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
     361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
     421 gggtcaaagt tggcgtttta ttattatagt cagtctagag atatactgag tcattaggga
     481 ctttccaatg ggttttgccc agtacataag gtcaataggg gtgaatcaac aggaaagtcc
     541 cattggagcc aagtacactg agtcaatagg gactttccat tgggttttgc ccagtacaaa
     601 aggtcaatag ggggtgagtc aatgggtttt tcccattatt ggcacgtaca taaggtcaat
     661 aggggtgagt cattgggttt ttccagccaa tttaattaaa acgccatgta ctttcccacc
     721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
     781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
     841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cacgcattct
     901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
     961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcat ggtgagcaag ggcgaggagc
    1021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
    1081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
    1141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
    1201 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
    1261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
    1321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
    1381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
    1441 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
    1501 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
    1561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
    1621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
    1681 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagatccac
    1741 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
    1801 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    1861 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    1921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    1981 ttaacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
    2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
    2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
    2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
    2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
    2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
    2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
    2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
    2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
    2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
    2581 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
    2641 acccaagctg gctagcgttt aaacgggccc tctagactcg agcggccgcc actgtgctgg
    2701 atatctgcag aattgatccg agctcggtac caagcttaag tttaaaccgc tgatcagcct
    2761 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
    2821 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
    2881 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
    2941 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt cgacttcgag
    3001 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    3061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    3121 ttatcatgtc tggatcgtct agcatcgaag atccaataac ttcgtatagc atacattata
    3181 cgaagttata agtagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    3241 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    3301 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    3361 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    3421 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    3481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    3541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    3601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    3661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    3721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    3781 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
    3841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    3901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    3961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    4021 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    4081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    4141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    4201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    4261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    4321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    4381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    4441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    4501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    4561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    4621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    4681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    4741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    4801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    4861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    4921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    4981 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    5041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    5101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    5161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    5221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    5281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    5341 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    5401 taaaaatagg cgtatcactc taggcaaaat agcaccctcc cgctccagaa caacatacag
    5461 cgcttcacag cggcagccta acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa
    5521 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag
    5581 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag
    5641 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa
    5701 tcgtcacttc cgttttccca cgttacgtaa cttcccattt taagaaaact acaattccca
    5761 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg
    5821 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtata
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