pET-22b(+)载体 pet22b质粒基本信息

pET-22b(+)质粒图谱 pet22b载体图谱和pET-22b(+)载体序列 pet22b质粒序列多克隆位点信息

pET-22b(+)质粒 pet22b载体简介

pET-22b(+)载体(货号：69744-3)携带有一个N端的pelB信号肽序列，能够将表达的目的蛋白定位于外周质腔，同时载体含有C端His标签。载体的单一的多克隆位点见上面的环状质粒图谱。注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。
T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。

pET-22b(+)质粒序列 pet22b载体序列

LOCUS       pET-22b(+)              5493 bp    DNA     circular     11-SEP-2008
SOURCE      
 ORGANISM  
COMMENT     This file is created by Vector NTI
           http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pET-22b(+)|
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
    promoter        complement(361..377)
                    /vntifkey="30"
                    /label=T7\promoter
    misc_signal     complement(224..289)
                    /vntifkey="87"
                    /label=pelB\sequence
    protein_bind    complement(140..157)
                    /vntifkey="31"
                    /label=His\tag
    terminator      complement(26..72)
                    /vntifkey="43"
                    /label=T7\terminator
    CDS             764..1843
                    /vntifkey="4"
                    /label=lac\I
    rep_origin      complement(3277..3277)
                    /vntifkey="33"
                    /label=ColE1\pBR322\origin
    CDS             complement(4038..4895)
                    /vntifkey="4"
                    /label=bla\(Ap)\sequence
    rep_origin      complement(5027..5482)
                    /vntifkey="33"
                    /label=f1\origin
    CDS             complement(334..358)
                    /vntifkey="4"
                    /label=lac\operator
BASE COUNT     1302 a      1456 c      1461 g      1274 t
ORIGIN
       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
     181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg
     241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc
     301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
     361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc
     421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
     721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
     781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
    1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
    1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
    1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
    1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
    1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
    1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
    1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
    1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
    1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
    1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
    1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
    1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
    1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca
    1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
    1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
    2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
    2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
    2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga
    2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
    2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
    2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
    2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
    2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
    2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
    2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
    2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
    2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
    2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
    2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
    3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac
    3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
    3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    3961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    4021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    4081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    4141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    4201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    4261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    4321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
    4381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    4441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    4501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    4561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    4621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    4681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    4741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    4801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    4861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    4921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    4981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt
    5041 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
    5101 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
    5161 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
    5221 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
    5281 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
    5341 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
    5401 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
    5461 aatgcgccgc tacagggcgc gtcccattcg cca
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