pGL4.43[luc2P/XRE/Hygro]

载体含有三份外来生物反应元件（XRE），该因子驱动荧光素酶报告基因luc2P（Photinus pyralis）的转录。luc2P是一种合成衍生的荧光素酶序列，具有人源化密码子优化功能，设计用于高表达和减少异常转录。luc2P基因含有hPEST，一种蛋白质不稳定序列，使luc2P蛋白水平比luc2更快响应转录诱导。载体主链包含一个氨苄青霉素耐药基因，用于选择大肠杆菌，以及一个具有抗性（hygromycin）的基因，使得稳定转染的哺乳动物细胞系得以选择。

Reporter vector PGL4.43[luc2P/XRE/Hygro], complete sequence

GenBank: JQ858513.1

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LOCUS       JQ858513                6067 bp    DNA     circular SYN 02-JUL-2012
DEFINITION  Reporter vector PGL4.43[luc2P/XRE/Hygro], complete sequence.
ACCESSION   JQ858513
VERSION     JQ858513.1
KEYWORDS    .
SOURCE      Reporter vector PGL4.43[luc2P/XRE/Hygro]
 ORGANISM  Reporter vector PGL4.43[luc2P/XRE/Hygro]
           other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6067)
 AUTHORS   McNamara,B.
 TITLE     Direct Submission
 JOURNAL   Submitted (28-MAR-2012) Scientific Communications, Promega
           Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USAFEATURES             Location/Qualifiers     source          1..6067
                    /organism="Reporter vector PGL4.43[luc2P/XRE/Hygro]"
                    /mol_type="other DNA"
                    /db_xref="taxon:1203007"     regulatory      105..258
                    /regulatory_class="terminator"
                    /note="transcriptional pause site"     regulatory      105..153
                    /regulatory_class="polyA_signal_sequence"
                    /note="synthetic poly(A) sequence"     primer_bind     207..226
                    /note="RVprimer3 binding site"     misc_feature    285..347
                    /note="XRE response element"     regulatory      393..423
                    /regulatory_class="promoter"
                    /note="minP promoter"     gene            456..2231
                    /gene="luc2P"
                    /note="luciferase"     CDS             456..2231
                    /gene="luc2P"
                    /codon_start=1
                    /transl_table=11
                    /product="luciferase"
                    /protein_id="AFM92227.1"
                    /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                    AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                    APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                    FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                    FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                    LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                    GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                    RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                    PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                    AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
                    LPMSCAQESGMDRHPAACASARINV"     regulatory      2271..2492
                    /regulatory_class="polyA_signal_sequence"
                    /note="SV40 late poly(A) signal"     regulatory      2540..2958
                    /regulatory_class="enhancer"
                    /note="SV40 enhancer and early promoter"     CDS             2983..4020
                    /note="hygromycin resistance"
                    /codon_start=1
                    /transl_table=11
                    /product="hygromycin phosphotransferase"
                    /protein_id="AFM92228.1"
                    /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                    GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                    LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                    HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                    EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                    SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                    TRPRAKEVGRV"     regulatory      4044..4092
                    /regulatory_class="polyA_signal_sequence"
                    /note="synthetic poly(A) sequence"     primer_bind     complement(4159..4178)
                    /note="RVprimer4 binding site"     rep_origin      4416..4452     gene            complement(5207..6067)
                    /gene="bla"     CDS             complement(5207..6067)
                    /gene="bla"
                    /note="AmpR"
                    /codon_start=1
                    /transl_table=11
                    /product="beta-lactamase"
                    /protein_id="AFM92226.1"
                    /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                    IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                    YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                    DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                    LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                    EIGASLIKHW"ORIGIN      
       1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
      61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
     121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
     181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
     241 gtgccagaac atttctctgg cctaactggc cggtacctga gctctgagtt ctcacgctag
     301 cagattgagt tctcacgcta gcagattgag ttctcacgct agcagatctc gaggatatca
     361 agatctggcc tcggcggcca agcttagaca ctagagggta tataatggaa gctcgacttc
     421 cagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga
     481 agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag
     541 ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg
     601 tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc
     661 gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct
     721 tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct
     781 acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga
     841 gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga
     901 tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga
     961 cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg
    1021 acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag
    1081 ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca
    1141 accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca
    1201 tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg
    1261 aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc
    1321 ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact
    1381 tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca
    1441 aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca
    1501 ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct
    1561 tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg
    1621 agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa
    1681 acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
    1741 acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg
    1801 tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg
    1861 tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac
    1921 acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg
    1981 ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca
    2041 agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg
    2101 ccgtgaattc tcacggcttc cctcccgagg tggaggagca ggccgccggc accctgccca
    2161 tgagctgcgc ccaggagagc ggcatggata gacaccctgc tgcttgcgcc agcgccagga
    2221 tcaacgtcta aggccgcgac tctagagtcg gggcggccgg ccgcttcgag cagacatgat
    2281 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
    2341 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
    2401 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
    2461 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt ttgcgtattg
    2521 ggcgctcttc cgctgatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
    2581 tggttagcta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
    2641 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    2701 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    2761 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    2821 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    2881 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    2941 ggcttttgca aaaagctcga ttcttctgac actagcgcca ccatgaagaa gcccgaactc
    3001 accgctacca gcgttgaaaa atttctcatc gagaagttcg acagtgtgag cgacctgatg
    3061 cagttgtcgg agggcgaaga gagccgagcc ttcagcttcg atgtcggcgg acgcggctat
    3121 gtactgcggg tgaatagctg cgctgatggc ttctacaaag accgctacgt gtaccgccac
    3181 ttcgccagcg ctgcactacc catccccgaa gtgttggaca tcggcgagtt cagcgagagc
    3241 ctgacatact gcatcagtag acgcgcccaa ggcgttactc tccaagacct ccccgaaaca
    3301 gagctgcctg ctgtgttaca gcctgtcgcc gaagctatgg atgctattgc cgccgccgac
    3361 ctcagtcaaa ccagcggctt cggcccattc gggccccaag gcatcggcca gtacacaacc
    3421 tggcgggatt tcatttgcgc cattgctgat ccccatgtct accactggca gaccgtgatg
    3481 gacgacaccg tgtccgccag cgtagctcaa gccctggacg aactgatgct gtgggccgaa
    3541 gactgtcccg aggtgcgcca cctcgtccat gccgacttcg gcagcaacaa cgtcctgacc
    3601 gacaacggcc gcatcaccgc cgtaatcgac tggtccgaag ctatgttcgg ggacagtcag
    3661 tacgaggtgg ccaacatctt cttctggcgg ccctggctgg cttgcatgga gcagcagact
    3721 cgctacttcg agcgccggca tcccgagctg gccggcagcc ctcgtctgcg agcctacatg
    3781 ctgcgcatcg gcctggatca gctctaccag agcctcgtgg acggcaactt cgacgatgct
    3841 gcctgggctc aaggccgctg cgatgccatc gtccgcagcg gggccggcac cgtcggtcgc
    3901 acacaaatcg ctcgccggag cgcagccgta tggaccgacg gctgcgtcga ggtgctggcc
    3961 gacagcggca accgccggcc cagtacacga ccgcgcgcta aggaggtagg tcgagtttaa
    4021 actctagaac cggtcatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
    4081 gttttttgtg tgttcgaact agatgctgtc gaccgatgcc cttgagagcc ttcaacccag
    4141 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
    4201 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
    4261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    4321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    4381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    4441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    4501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    4561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    4621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    4681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    4741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    4801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    4861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    4921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    4981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    5041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    5101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    5161 taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga
    5221 tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc
    5281 ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga
    5341 tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga
    5401 gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct
    5461 gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg
    5521 ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc
    5581 agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag
    5641 ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag
    5701 cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt
    5761 actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt
    5821 ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc
    5881 gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc
    5941 ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg
    6001 caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga
    6061 tgctcat//