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人cDNA文库、人ORF克隆、基因

发布时间:2026-04-24 来源:

  #人cDNA文库、人ORF克隆、基因
        人cDNA文库,包含了15,000个人基因ORF全长克隆,您在科研中,无需通过合成得到基因,节约成本和时间。
       
        人基因开放阅读框(ORF)库(Human Open Reading Frame library)包含约15,000个人基因ORF克隆,其中包括已知的基因约12,000种。本人基因ORF库的每一种质粒都来自于单克隆,并且每一个ORF都经过了测序验证。后续使用Gateway系统或PCR反应即可非常方便地克隆目的基因到新的表达载体中。
      
        本人基因ORF库的克隆在供体载体(Donor vector) pDONR223中。
      
       ORF克隆是指起始密码子和终止密码子之间的编码序列,而不包含mRNA的5'和3'端的非编码区(untranslated region, UTR)。本ORF库是通过mRNA反转录获得的编码蛋白质的cDNA,再通过Gateway技术的BP重组反应克隆到pDONR223载体中得到入门载体。为了便于后续利用Gateway系统添加适当的3'标签,所有的克隆都无终止密码子。
      
       整个文库出售,非单条基因,欢迎咨询

另有如下文库可供选择:

货号基本信息
TY-1112A/B人全基因组敲除质粒文库A/B(单质粒系统/one-vector system)
Addgene:Library#1000000048;
Human CRISPR Knockout Pooled Library(GeCKO v2)
TY-1113A/B人全基因组敲除质粒文库A/B(双质粒系统/two-vector system)
Addgene:Library#1000000049;
Human CRISPR Knockout Pooled Library(GeCKO v2)
TY-1114人全基因组质粒敲除文库(Brunello);(双质粒系统/two-vector system)
Library #73178;Human CRISPR Knockout Pooled Library (Brunello)
TY-1115A/B小鼠全基因组敲除质粒文库A/B(单质粒系统/one-vector system)
Addgene:Library#1000000052;
Mouse CRISPR Knockout Pooled Library(GeCKO v2)
TY-1116A/B小鼠全基因组敲除质粒文库A/B(双质粒系统/two-vector system)
Addgene:Library#1000000053;
Mouse CRISPR Knockout Pooled Library(GeCKO v2)
TY-1117人表观遗传敲除质粒文库(单质粒系统/one-vector system)
Library#162256;Human Epigenetic Knockout Library
TY-1118人RNA结合蛋白敲除质粒文库(单质粒系统/one-vector system)
Library#141438;
Yeo Lab RNA-Binding Protein Pooled CRISPR Knockout Library
TY-1119小鼠代谢基因敲除质粒文库(单质粒系统/one-vector system)
Library#160129;Mouse CRISPR Metabolic Gene Knockout Library
TY-1120人脑cDNA基因质粒文库
TY-1111人15000条全长cDNA文库
TY-1121小鼠脑cDNA基因质粒文库
TY-1122大鼠脑cDNA基因质粒文库
TY-1123A/B人全基因组激活质粒文库A/B(双质粒系统/two-vector system)
Library#92379;Library#92380;
Human CRISPR Activation Pooled Library (Calabrese P65-HSF)
TY-1124小鼠全基因组激活质粒文库(SAM-3 plasmid system)
Library#1000000075;
Mouse CRISPR Activation Library (SAM-3 plasmid system)
TY-1125人长非编码RNA(lncRNAs)敲除pgRNA文库
Library#89640;
Human paired-guide RNA (pgRNA) Library for long non-coding RNAs (lncRNAs)
TY-1126人代谢基因敲除文库;Library#110066;
Human CRISPR Metabolic Gene Knockout Library
TY-1127人激酶敲除文库;Library#117725;
Human Kinase Domain-Focused CRISPR Knockout Library
TY-1128小鼠心脏转录调控因子敲除文库;Library#138015;
Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library
TY-1129人DNA结合域敲除文库;Library#123334;
Human DNA Binding Domain-Focused CRISPR Knockout Library
TY-1130人lncRNA剪接位点敲除文库;Library#119977;
Human lncRNA Splicing-targeting CRISPR Library
TY-1131人溶质转运基因敲除文库;Pooled Library #132552;
Superti-Furga Lab Human SLC Knockout Library
TY-1132小鼠溶质转运基因敲除文库;Pooled Library #141436;
Superti-Furga Lab Mouse SLC Knockout Library
TY-1133人泛素化相关蛋白敲除文库;Pooled Library #174592;
Human ubiquitination-related proteins CRISPR KO library
TY-1134人CRISPR lncRNA激活文库(三质粒系统/SAM-3 plasmid system)
Human CRISPR lncRNA Activation Pooled Library (SAM-3 plasmid system);Pooled Library #1000000106;
TY-1135人全基因组激活质粒文库(三质粒系统/SAM-3 plasmid system)
Pooled Library #1000000057, #1000000074;
Human CRISPR Activation Library (SAM-3 plasmid system)
TY-1136人膜蛋白激活文库;Pooled Library #113345;
Wright Human Membrane Protein Activation Library
TY-1137人溶质转运基因激活文库;Pooled Library #132561;
Superti-Furga Lab Human SLC Activation Library
TY-1138Human CRISPR Activation Pooled Library ( P65-HSF)
(人全基因组激活文库)
TY-1139Mouse CRISPR Activation Pooled Library ( P65-HSF)
(小鼠全基因组激活文库 )
TY-1140Human CRISPR Inhibition Pooled Library
(人全基因组CRISPRi文库)
TY-1141Mouse CRISPR Inhibition Pooled Library
(小鼠全基因组CRISPRi文库)
TY-1142Human CRISPRi Non-coding Libraries
(人类细胞非编码基因CRISPRi文库)
TY-1143E.coli Genome-wide Inhibition Library
(大肠杆菌全基因组CRISPRi文库)